Coding

Part:BBa_K1903009:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


RFP-IntN DnaB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is based on a RFP fused to the Intein N terminal from SSp DnaB expressed as a fusion protein.

Note: The sequence has a 6X Histag before the RFP E1010 part but it is not in the Open Reading Frame, for further uses needs to be redesign, for example, adding a methionine codon before the 6X Histag sequence.

Source

This BioBrick is formed with the RFP BBa_E1010 and the Intein N terminal from Ssp DnaB BBa_K1903019.

References